Anonymous
Anonymous asked in 科學及數學化學 · 1 decade ago

化學高手幫幫忙~ about HPLC

點樣用由HPLC 測出既peak 去決定所測物質( 一種藥丸) 既content (mg) ?? 有冇算式去計 ?

http://farm3.static.flickr.com/2088/2085426963_f6a...

(standard)

http://farm3.static.flickr.com/2204/2085426959_0d5...

( my sample)

1 Answer

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  • ?
    Lv 4
    1 decade ago
    Favorite Answer

    Assume the amount of the standard is y mg and the condition is no change during these 2 tests

    Then the amount of your sample is

    C (your sample) = C (standard) x Peak area (your sample) / Peak area (standard)

    = y x 4147 / 272.22

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