Eurika asked in 科學動物學 · 1 decade ago

有人在做masson's trichrome嗎

我目前在染的是肺的切片

都是按照protocol做的

但是紅色的部分都染不上

有人可以告訴我原因嗎?

1 Answer

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  • 1 decade ago
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    Masson Red stain for 2mins.

    leave it longer. 然後用PMA wash our the collagen, make sure only the collagen is washed out, not the muscles, otherwise, your staining will be not good. 然後用 Methyle blue, 也是一樣是2mins.

    你Masson red 用多久, Methyle blue 就用多久. 比較要注意的是PMA的地方, 需要Microscopy control.

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