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泥鰍 asked in 社會與文化語言 · 1 decade ago

請有關醫學方面的高手幫我翻譯一下這段文章

GFP+ NEPs were kindly provided by Dr. Gang Lu (The Chinese

University of HongKong, HongKong, China). GFP+ NEPs

were harvested from enhanced GFP transgenic mice (green

mice), in which the expression of GFP is under the control of

a chicken -actin promoter. The method for preparing NEPs

used in this study, has been previously described in another of

our studies [16]. Briefly, Rostral regions of the neural tubes

were dissected from GFP+ transgenic embryos of embryonic

day 11 (E11, 11th day of gestation). NEPs suspensions were

passed through a series of D-Hank’s rinses, then digested with

0.125% trypsin for 10 min at 37 ◦C, and washed in D-Hank’s

solution containing 0.004% DNase. The dissociated cells were

suspended in N2 medium, a defined DMEM/F12-based medium

(Life Technologies) supplemented with B27 (Gibco), human

recombinant epidermal growth factor (EGF) (20 ng/ml; Sigma)

and human recombinant basic fibroblast growth factor (FGF)

(20 ng/ml; Sigma). Five mililiters of the NEP cell suspension

at a density of 3×104 cells/ml were plated into each well of

uncoated six-well plates and incubated at 37 ◦C in a humidified

incubator with 5% CO2.

4 Answers

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  • 1 decade ago
    Favorite Answer

    博士和善提供了GFP+ NEPs 幫陸(香港,中國,香港的ChineseUniversity)。 GFP+ NEPswere從被提升的GFP斬獲轉基因的老鼠(greenmice),其中GFP的表示是在控制ofa雞肉下 -actin 發起者。 方法適合準備NEPsused在這研究內,已經被以前在另一ofour內描述研究 [16]. 短暫,神經的tubeswere的Rostral 地區從GFP+ 解剖embryonicday 11(E11,醞釀的第11天)的轉基因的胎兒。 透過一系列D-漢克沖洗的NEPs停職werepassed, 10一瞬間37的然後消化的with0.125% trypsin ◦C,和在D-Hanksolution 包含0.004%的DNase裡洗。 用N2 媒介的被分離的小屋weresuspended, 用B27(Gibco),humanrecombinant表皮炎增長系數(EGF)補充的一確定的DMEM /基于F12的媒介(生命技術)(20 ng/ml; 總和)和人 recombinant 基本增長系數fibroblast(FGF)(20 ng/ml; 總和)。 密度的3 cells/ml 04的被鍍的5 mililiters的suspensionat 細胞那些NEP的進每好ofuncoated 6 健康盤子並且對37孵化 ◦在用5%的CO2的humidifiedincubator裡的C。

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    6 years ago

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  • 1 decade ago

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  • 1 decade ago

    答:

    GFP+ NEPs 是親切地由組織博士 Lu 提供(華人

    香港大學、香港,中國). GFP+ NEPs

    從可提高的 GFP 基因轉殖的老鼠被收割(綠色的

    老鼠), 在哪一個 GFP 的表達在控制之下

    一個小雞-肌動蛋白的促進者。 準備 NEPs 的方法

    在這研究使用,先前已經被在另外一描述

    我們的研究〔16]. 簡短地,神經管的嘴區域

    從 GFP 被解剖+基因轉殖胚胎胚胎的

    第 11 日子 (E11, 第 11個日子

    Source(s): 我+字典
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