what is the definition of noise in chromatogram and why does it happen?can somebody help me?
- 1 decade agoFavorite Answer
well it a noise is basically a background signal that oscillates (not necessary periodically) without any contribution of the analysed components.
There are many reason why noise could appear in a chromatogram and it also depends on the type of detector that you use for your chromatography equipment.
if you are using FID (flame ionisation detector) you might also ionise air (which mostly contain N2 and O2) and as their composition might fluctuate a bit this will contribute to noise signal in your chromatograph.
The other cause might come from the carrier substance. Carrier substances (gas or liquid) should be pure and inert substances but as this is impossible impurities of these carrier substances might also contribute to noise signal.
The other cause might also due to the strongly sensitive detector that you are using in the chromatography as a slight fluctuation in flow or composition could trigger instable signal reading.
These causes might also occur in different detector type such TCD (thermal conductivity detector), PID (photo ionisation detector), Electrolytic Conductivity Detector (ELCD) and so on.
hope that would help mate!