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ㄚ玟 asked in 科學生物學 · 1 decade ago

紅螢光蛋白的吸收範圍

我需要紅螢光蛋白(RFP)的吸收範圍

不是細胞喔~!

是單純紅螢光蛋白的吸收範圍(波長)

請告訴我資料來源或是哪篇paper

感激不盡~!

1 Answer

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    Lv 5
    1 decade ago
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    範圍最小450nm 範圍最大650nm 激發最高的吸收峰出現在558nm 應該是這樣 詳情請見參考網站 還有附圖

    Red Fluorescent Proteins

    The first coral-derived fluorescent protein to be extensively utilized was derived from Discosoma striata and is commonly referred to as DsRed. Once fully matured, the fluorescence emission spectrum of DsRed features a peak at 583 nanometers whereas the excitation spectrum has a major peak at 558 nanometers and a minor peak around 500 nanometers. Several problems are associated with using DsRed, however. Maturation of DsRed fluorescence occurs slowly and proceeds through a time period when fluorescence emission is in the green region. Termed the green state, this artifact has proven problematic for multiple labeling experiments with other green fluorescent proteins because of the spectral overlap. Furthermore, DsRed is an obligate tetramer and can form large protein aggregates in living cells. Although these features are inconsequential for the use of DsRed as a reporter of gene expression, the usefulness of DsRed as an epitope tag is severely limited. In contrast to the jellyfish fluorescent proteins, which have been successfully used to tag hundreds of proteins, DsRed conjugates have proven much less successful and are often toxic.

    看圖比較清楚

    圖片參考:http://www.fileupyours.com/files/77921/yahooknow/f...

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