asked in 科學化學 · 1 decade ago

pH-tuned enzyme 是做啥的,有啥功用?

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pH-tuned enzyme是做啥的,有啥功用呢?!

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  • Tiger
    Lv 5
    1 decade ago
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    pH-tuned enzyme: Enzyme with the activity in aqueous media is controlled by the choice of pH. Enzymes function best at a given optimum pH and temperature. For enzyme catalysis in organic media, pH is meaningless due to the absence of a continuous aqueous phase and therefore ionisation-state of the protein is considered in replacement of pH. The pH of a solution can have several effects of the structure and activity of enzymes.For example, pH can have an effect of the state of ionization of acidic or basic amino acids. Acidic amino acids have carboxyl functional groups in their side chains. Basic amino acids have amine functional groups in their side chains. If the state of ionization of amino acids in a protein is altered then the ionic bonds that help to determine the 3-D shape of the protein can be altered. This can lead to altered protein recognition or an enzyme might become inactive.Changes in pH may not only affect the shape of an enzyme but it may also change the shape or charge properties of the substrate so that either the substrate connot bind to the active site or it cannot undergo catalysis.In geneal enzyme have a pH optimum. However the optimum is not the same for each enzyme.For example in the figure below is represented a situation inwhich two different enzymes might have very different pH optima. The one depicted by the green curve might represent the pH optimum for the enzyme pepsin which degraded proteins (protease) in the vert acidic lumen of the stomach. The second curve (in red) might represent the enzyme carbonic anhydrase that works in the neutral pH of your cytosol. 

    圖片參考:http://www.emc.maricopa.edu/faculty/farabee/biobk/...

    Plot of enzyme activity as a function of pH for several enzymes. Note that each enzyme has a range of pH at which it is active as well as an optimal pH at which it is most active.

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