Ken asked in 健康疾病與處置傳染病 · 1 decade ago

Infectious Mononucleosis(IM)?

How can IM antibodies be distinguished from other heterophile antibodies?(Please in detail replied....thank you~!)

1 Answer

Rating
  • micky
    Lv 5
    1 decade ago
    Best Answer

    Infectious mononucleosis is most frequently encountered among children and young adults. The diagnosis of IM is based on clinical, hematological and serological findings. The first serological test described was heterophile antibody assay developed by Paul and Bunnel. The assay was later modified by Davidsohn by introducing guinea pig kidney powder absorption to prevent interference of the Forssmann antibody. Ever since the development of Paul-Bunnel-Davidsohn test, the presence of specific heterophile antibody is still considered as diagnostic of IM. It has been shown that only the IgM fraction of PB antibody positive serum from an IM patient reacted specifically by indirect membrane fluorescence test with EBV producer lymphoblastoid cell lines. It suggested that the PB antigen on the surface of EBV induced the production of IM specific heterophile antibody.Paul-Bunnel-Davidsohn test : After inactivating the sera, both presumptive and differential tests were performed in parallel as described by Lennette et al.(i) Presumptive test : Serial two fold dilutions of 0.1ml sera, in PBS pH 7.2, were made ranging from 1:10 to 1:160 in tubes and each dilution was mixed with equal volume of 1% sheep erythrocytes. The tubes were incubated for 3hours at 37°C in water bath before taking the readings. (ii) Differential test : The sera were absorbed with 2% guinea pig kidney powder prepared according to the method of Buescher and the rest of the test was carried out in the same way as for presumptive test.A heterophile agglutinin titre of 1:10 or more in absorbed serum samples was considered to be positive for diagnosis of infectious mononucleosis.

    Source(s): google
Still have questions? Get your answers by asking now.