asked in 科學生物學 · 1 decade ago

關於” CAT report gene”?!

想請問

關於分子生物技術上面常使用到的\"CAT report gene\"

其原理和作用機轉 感謝.........

1 Answer

Rating
  • micky
    Lv 5
    1 decade ago
    Favorite Answer

    A very popular reporter gene and protein has been chloramphenicol acetyltransferase or CAT. This is a bacterial gene that evolved to neutralize the antibiotic chloramphenicol. To neutralize it, CAT transfers acetyle groups to chloramphenicol and thus changes its shape into a harmless form. Molecular biologists were quick to recognize this enzyme could be used as a reporter protein in eukaryotic cells. If you wanted to determine when a promoter were activated, you could place the promoter of interest upstream of the CAT coding sequence and either measure mRNA or protein production. Eric Davidson measured both mRNA and protein production in the chapter on single gene circuitry.

    看圖吧!!!

    http://www.bio.davidson.edu/Courses/genomics/metho...

Still have questions? Get your answers by asking now.