star asked in 科學生物學 · 1 decade ago

如何根據Real-time PCR結果計算與分析基因表現

如何根據Real-time PCR結果計算與分析基因表現?

Ex: CT值如下:sample 1-internal control:1.8 ;A基因:3

sample 2-internal control:1.7;A基因:4

什的表現量高?高多少?比例又是多少??

1 Answer

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  • Anonymous
    1 decade ago
    Favorite Answer

    A genes expression:

    sample 1 : sample 2 = 1 : 0.4665 or 2.144 : 1

    sample 1 比 sample 2 高 2.144倍

    Source(s): Kenneth J. Livak and Thomas D. Schmittgen, METHODS 25, 402–408 (2001)
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