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凱如 asked in 社會與文化語言 · 1 decade ago

好心人幫幫我翻譯~~好像有關生物技術的...!!

In this paper we report that the inclusion of heatresistant RecA protein from a thermophilic bacteria, Thermus thermophilus ,and its cofactor (ATP) in PCR effectively eliminates non-specific PCR products. The effect of RecA protein, which catalyzes pairing between homologous DNA molecules with great fidelity in genetic recombination, is due to its promotion of precise priming in PCR(i.e. priming at sites where the primer sequence is completely complementary to that of the target sequence).In addition, the RecA protein substantially reduces the primer concentration required for PCR. These experimental results have led to the realization of multiplex PCR, which involves PCR for multiple sites in the same reaction mixture. We were able to successfully perform multiplex PCR with over a dozen reactions without affectiong the amplification pattern of the PCR products.

已修改!!!

幫一下吧!!

謝謝各位好心人土~~~>_<

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  • 1 decade ago
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    這個紙,我們,報告排除實際上,在PCR的高溫細菌,Thermus thermophilus,波及的來自那個補助因子(ATP)的heatresistant RecA蛋白質的蘊藏不被特別指定的PCR產品。 對遺傳基因的「再-組合」有極好的信義的以狀態促進同源的DNA分子之間的組合的RecA蛋白質的效果PCR(在即,入門書系列完全補充靶子排列的東西的站點的準備).是因為In加添能放的正確的準備的銷售促進,RecA蛋白質壓倒具有實際內容地PCR必要的入門書集中。 這些的實驗結果與multiplexPCR的實現相連了。(PCR關乎PCR同樣的反應混合物的複數的站點)。 我們能順利地實行沒有放大按照形式製作的PCR產品的affectiong有1打以上的反應的multiplexPCR就。

    http://excite.co.jp/world/chinese/   這網站翻譯的 

    Source(s): 希望幫的上忙
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  • Anonymous
    6 years ago

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