怡文 asked in 科學生物學 · 1 decade ago

請問HE stain的protocol

請問HE stain的protocol,將組織去下切片之後,要如何處理?

Update:

請問,第6.7.8點,是用多少濃度的酒精?

2 Answers

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  • 1 decade ago
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    常規染色,Hematoxylin & Eosin Y stain, HE stain◎固定液:10%neutral buffered formalin◎切片厚度:3-6mm paraffin sections◎染色步驟:1. Xylene 5 mins2. Xylene 10 mins3. Xylene 10 mins4. 100%Alcohol 1 mins5. 100%Alcohol 1 mins6. Alcohol 30 secs7. Alcohol 30 secs8. Alcohol 30 secs9. 水洗(water wash)2 mins10 Hematoxylin 5 mins11. 水洗(water wash)25 mins12. Eosin 3 mins13. 80%Alcohol 20 secs14. 90%Alcohol 20 secs15. 95%Alcohol 20 secs16. 100%Alcohol 30 secs17. 100%Alcohol 30 secs18. Xylene & 100%Alcohol 2 mins19. Cresol solution 1 min20. Xylene 1 min21. Xylene 1 min22. Xylene 1 min23. 封片◎染色結果:細胞核、細菌        blue細胞質、間質、各種纖維類        light red to red

    Source(s): TMC
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  • 1 decade ago
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