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Before sacrificing mammalian experimental subjects (such as mouse and rats), we simply anesthetize them with sodium pentobarbital (100 mg/kg) and then either break their neck or chop off their head to sacrifice them. Note that these are for animals you will need to savage the tissue for your experiments.
If you are talking about just sacrificing animals that are useless for your experiments, just overdose them in carbon monoxide chambers.Source(s): I am a graduate student in biology