fly asked in 科學動物學 · 2 decades ago

請問DTAF這種螢光染劑的功用

請問有人知道DTAF和EOSIN這種染劑的功用嗎?且EOSIN可以用在螢光顯微鏡下嗎

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  • 2 decades ago
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    既然能問這種問題,那就先假設您有辦法念paper囉!

    DTAF:

    5-(4,6-dichlorotriazinyl)aminofluorescein (5-DTAF) is a reactive dye with absorption/emission maxima of ~492/516 nm. Dichlorotriazines readily modify amines in proteins, and are among the few reactive groups that are reported to react directly with polysaccharides and other alcohols in aqueous solution, provided that the pH is >9 and that other nucleophiles are absent.

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    如果是你實驗用的,看跟哪一家買的,去他們母公司的網站會又詳細的介紹、用法,記得要看 MSDS 。自己查出來的資料才是自己的。如果是paper上的,看看他後面會有個小括號,裡面寫的就是公司,去查一查。

    J Immunol Methods. 1976;13(3-4):305-20.

    Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG.

    Blakeslee D, Baines MG.

    Dichlorotriazinylaminofluorescein (DTAF), the product of the reaction of aminofluorescein with cyanuric chloride, is an effective reagent for conjugating fluorescein to immunnoglobulins. DTAF has absorption and emission properties nearly identical to fluoresceinisothiocyanate (FITC) and DTAF and FITC-labelled antibodies are similar in terms of preparation and specificity of immlnofluorescence. However, DTAF is superior to FITC with regard to cost, purity and stability. Also, DTAF-labelled rabbit IgG conjugates can by quickly and efficientyly fractionated by simple ammonium sulfate precipitation procedures to yield preparations free of both over- and under-conjugated material. In addition, over-conjugated protein can be readily removed from DTAF:IgG conjugates by appropriate adjustment of pH and temperature.

    EOSIN

    1. A red crystalline powder, C20H8O5Br4, used in textile dyeing and ink manufacturing and in coloring gasoline.

    2. The red sodium or potassium salt of this powder, used in biology to stain cells.

    發音:http://www.thefreedictionary.com/eosin

    Eosin is vital in medicine and biological science to show details in cells and microorganisms. It highlights cell granules and nuclei, and mast cells (cells that create other cells). Eosin demonstrates the presence of viruses borne by mosquitoes, or early necrobiotic changes. It is used to characterize tissue cells, protozoans and bacteria. Eosin's most important medical uses are in blood and bone-marrow testing.

    Industry uses Eosin's strong staining properties to efficiently dye nylon, silk, and wool. That thin red stripe on your notebook paper is probably Eosin.

    他當然可以用在螢光顯微鏡下,不過只有亮視野看得清楚,螢光很弱。

    The reactive eosin (2',4',5',7'-tetrabromofluorescein) and erythrosin (2',4',5',7'-tetraiodofluorescein) dyes are usually not chosen for their fluorescence properties — the fluorescence quantum yield of eosin is typically only about 10–20% that of fluorescein, and erythrosin is even less fluorescent — but rather for their ability to act as phosphorescent probes or as photosensitizers.

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