Yahoo Answers is shutting down on May 4th, 2021 (Eastern Time) and beginning April 20th, 2021 (Eastern Time) the Yahoo Answers website will be in read-only mode. There will be no changes to other Yahoo properties or services, or your Yahoo account. You can find more information about the Yahoo Answers shutdown and how to download your data on this help page.

Derek asked in 科學動物學 · 2 decades ago

請問一下Carmine的染色機制

在做細胞染色體行為觀察時用的carmine染劑的染色機制?

1 Answer

Rating
  • ?
    Lv 5
    2 decades ago
    Favorite Answer

    Carmine(洋紅):一種深紅色色料,源自於墨西哥或中美洲的甲蟲類名叫燕脂蟲中枯了的翅膀提煉,

    染色體內部的物質主要為去氧核糖核酸(deoxyribonucleic acid,DNA),其次為一種鹼性的核蛋白(histone),二者組成一種複雜的化合物。另外染色體尚包含一些其他的蛋白質(如酸性核蛋白)及核糖核酸(ribonucleic acid, RNA)。但主要的物質,也就是染色體的骨幹仍然是DNA。其內部的鹼性物質可被一些酸性染料 (如 aceto carmine)染色,其內部的酸性物質可被一些鹼性染料(如 basic fuchsine)染色。

    Aceto-Carmine Chromosome Stain Recipe

    There are several versions of this stain. An iron containing stain is often used because of the darker bluish-red color produced.

    Non-iron version

    Heat a solution of 45% acetic acid (45ml glacial acetic acid/55ml of distilled water) to boiling.

    Add 0.5g of Carmine and continue heating for 15-20 minutes while stirring.

    Cool resulting solution.

    Filter to remove any precipitate.

    Iron containing version

    Heat a solution of 45% acetic acid (45ml glacial acetic acid/55ml distilled water) to boiling. ALWAYS use caution and wear personal protective equipment when in the laboratory with concentrated acids (or any other time)!

    Add 0.5g of Carmine and continue heating for 15-20 minutes while stirring.

    Cool resulting solution.

    Filter to remove any precipitate.

    Separately make a solution of 45ml glacial acetic acid/55ml distilled water/5g Ferric oxide).

    Slowly (dropwise) add the Ferric oxide solution to 50ml of the Carmine solution until a precipitate starts to appear.

    Promptly add 50ml of Carmine solution to the titrate mixture.

    Filter to remove any precipitate.

    Cautions: ALWAYS use caution and wear personal protective equipment when in the laboratory with concentrated acids (or any other time)! Always add acid slowly into water and do not add water into acid!

Still have questions? Get your answers by asking now.