Anonymous
Anonymous asked in 科學動物學 · 2 decades ago

為什麼 RNA 比 dsDNA 更好 degrade?

Why is RNA more easily degraded compared to dsDNA?

那在一般實驗裡,要如何 minimise RNA degradation? 除了所有東西都要消毒和手套要一直換外

Update:

weekend play u did not answer my questions

beside.. RNA is easier to be contaminated, due to the large amount of nucleases on our hand etc...

and i thinks RNA is eaiser to be degreaded because it's single strand.. (just asked someone)

2 Answers

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  • Anonymous
    2 decades ago
    Favorite Answer

    RNA 比 dsDNA 更好 degrade是因為:

    1.DNA 是去氧核糖核酸而RNA是核糖核酸,RNA比DNA多了氧基(-OH vs -H),就是因為這個-OH所以在化學上更容易與其他分子鍵結而產生作用.

    2.有沒有double strand非主要因素,因為RNA也會自行配對形成穩固的結構,此點為與大部分的人認知不同喔!!!

    3.最主要的為此點:RNAase,非常的頑固---高溫高壓滅菌與一些普通化學藥劑都沒辦法將其破壞,因此在一般實驗裡,要如何 minimise RNA degradation,所有東西都要消毒(DEPC處理)和要很小心的操做實驗(人體與空氣中充滿了RNAase).

    Source(s): 自己
  • ?
    Lv 6
    2 decades ago

    RNA 比 dsDNA 更好 degrade是因為RNA 是核糖核酸

    DNA 是"去氧"核糖核酸

    所以DNA容易被污染

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