What is Chromatography?

How is chromatography done?

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  • 1 decade ago
    Best Answer

    Chromatography is a method that Chemists use to determine the composition of a substance. There are several types of chromatography including gas and gas/liquid.

    In general the substance are put under a certain test in which each element reacts differently to. This allows the reaction of the substance to be compared with known reactions of elements to help determine the composition, and in some methods the ratio of elements in the substance.

    Another similar method involves running the chromatograph test with substances believed to be the compoisition. The tester can them compare the response of the expected substances against the test substance.

    Hope this helps :-)

    Source(s): University Chemistry studies
  • 1 decade ago

    Chromatography is a family of analytical chemistry techniques for the separation of mixtures. It involves passing the sample, a mixture which contains the analyte, in the "mobile phase", often in a stream of solvent, through the "stationary phase." The stationary phase retards the passage of the components of the sample. When components pass through the system at different rates they become separated in time, like runners in a mass-start foot race. Each component has a characteristic time of passage through the system, called a "retention time." Chromatographic separation is achieved when the retention time of the analyte differs from that of other components in the sample.

    A chromatograph takes a chemical mixture carried by liquid or gas and separates it into its component parts as a result of differential distributions of the solutes as they flow around or over a stationary liquid or solid phase. Various techniques for the separation of complex mixtures rely on the differential affinities of substances for a gas or liquid mobile medium and for a stationary absorbing medium through which they pass; such as paper, gelatin, or magnesium silicate gel.

    Analytical chromatography is used to determine the identity and concentration of molecules in a mixture. Preparative chromatography is used to purify larger quantities of a molecular species. Most of the following refers to analytical chromatography.

  • 1 decade ago

    Chromatography is a family of analytical chemistry techniques for the separation of mixtures. It involves passing the sample, a mixture which contains the analyte, in the "mobile phase", often in a stream of solvent, through the "stationary phase." The stationary phase retards the passage of the components of the sample. When components pass through the system at different rates they become separated in time, like runners in a mass-start foot race. Each component has a characteristic time of passage through the system, called a "retention time." Chromatographic separation is achieved when the retention time of the analyte differs from that of other components in the sample.

    A chromatograph takes a chemical mixture carried by liquid or gas and separates it into its component parts as a result of differential distributions of the solutes as they flow around or over a stationary liquid or solid phase. Various techniques for the separation of complex mixtures rely on the differential affinities of substances for a gas or liquid mobile medium and for a stationary absorbing medium through which they pass; such as paper, gelatin, or magnesium silicate gel.

  • 1 decade ago

    it is a detailed process. u have chromatography columns . which are like tubes which are filled with gels. it is loaded with buffers abd then the require sample to be separated is loaded into it.

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  • 4 years ago

    Thin Layer Chromatography (TLC) is similar to Paper except rather than using a stationery phase of paper it uses a thin layer of adsorbent (silica gel or cellulose) on a flat, unreactive substrate. It is therefore better as it runs faster and has clearer separations

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