What is the difference between septate hyphae and nonseptate hyphae?

Answer 1

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Septate hyphae have cross-walls (called septa) that divide the cytoplasm into segments. They usually aren't complete divisions, so cytoplasm can still flow between them, but they're enough to keep major cellular components from wandering around too much.

Nonseptate hyphae are just long, continuous tubes of cytoplasm. There are no septa that roughly distinguish one cell from another.